Alaaddin Bulak Arpat

Highlights • Global profiling of mRNA decay pathways and aberrant translation events in mESCs • XRN1 mediates mRNA turnover, whereas SKIV2L acts widely in translation surveillance • AVEN interacts with ribosomes and the Ski complex and counteracts ribosome stalling • Histone mRNAs, uORFs, and small ORFs are key targets of SKIV2L and AVEN Summary RNA decay is crucial for mRNA turnover and surveillance and misregulated in many diseases.

], we had used ribosome profiling [ ] (RPF-seq) on a time series of organs collected from mice sacrificed every 2 h over the 24-h day (12 timepoints in duplicate; Fig. ). To generate a complementary dataset from a second organ, we chose the kidneys from the same cohort of animals. Liver and kidney express thousands of genes in common [ , ], thus providing a particularly suitable setting for a cross-organ comparison of gene expression.