Alejandro Mena Acra

Submitted: 30 December 2025 You are already at the latest version Background/Objectives: Many products that claim to have anti-aging effects have been reported, but their relative potency is not clear. In this study, in vitro replicative lifespan extension (RLE) activity of various groups of physiologically active substances was compared by updated “overlay method”.

Keywords: Anti-aging replicative life span quantification “overlay” method hormones antioxidants plant extracts X-ray quercetin Introduction The increase in the aged population has emerged as a matter of great concern. Over the past 60 years, the proportion of the world population aged 60 and over has increased year by year. In the next 25 years, the aged group is estimated to rise from 0.8 to 2 billion (1).

Key Words: UVC protection macrophage activation alkaline extract pine seed shell There are many useful medicinal plants in nature. In folklore, home-made concoctions of medicinal plants have been used to treat diseases in daily life. In fact, most Japanese traditional herbal (‘Kampo’) medicines and their constituent plant extracts are prepared by hot-water extraction (1, 2).