Aliaksandr Halavatyi

Abstract Mitotic spindles are dynamically intertwined with the cytoplasm they assemble in. How the physicochemical properties of the cytoplasm affect spindle architecture and size remains largely unknown. Using quantitative biochemistry in combination with adaptive feedback microscopy, we investigated mitotic cell and spindle morphology during neural differentiation of embryonic stem cells.

Published In Science Translational Medicine Volume 16 | Issue 778 December 2024 Article versions Submission history Received: 19 June 2023 Resubmitted: 25 April 2024 Accepted: 1 November 2024 Permissions Request permissions for this article. Acknowledgments We thank C. Stolp for providing helpful assistance in ensuring a smooth availability and processing of human lung tissue. We thank C. A. Staab-Weijnitz (CPC Munich) for advice on improving mass spectrometric coverage of collagens in our data.

Abstract Subnuclear compartmentalization has been proposed to play an important role in gene regulation by segregating active and inactive parts of the genome in distinct physical and biochemical environments. During X chromosome inactivation (XCI), the noncoding Xist RNA coats the X chromosome, triggers gene silencing and forms a dense body of heterochromatin from which the transcription machinery appears to be excluded.