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High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue

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By Yang Liu, Mingyu Yang, Yanxiang Deng, Graham Su, Toma Tebaldi, Di Zhang, Dongjoo Kim, Yang Xiao, Rong Fan, Archibald Enninful, Cindy C. Guo, Zhiliang Bai, Eileen Norris, Alisia Pan, Jiatong Li, Stephanie Halene
| ScienceDirect Verified
Highlights • Deterministic barcoding in tissue enables NGS-based spatial multi-omics mapping • DBiT-seq identified spatial patterning of major tissue types in mouse embryos • Revealed retinal pigmented epithelium and microvascular endothelium at cellular level • Direct integration with scRNA-seq data allows for rapid cell type identification Summary We present deterministic barcoding in tissue for spatial omics sequencing (DBiT-seq) for co-mapping of mRNAs and proteins in a formaldehyde-fixed...
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