Andrew Field

Method details Generation of INTS11Halo mESC cells To generate repair templates for CRISPR, homology arms corresponding to genomic regions surrounding the INTS11 start codon were cloned into pGEM-3Z (a gift of Danette Daniels and Elizabeth Caine, Promega) using Gibson assembly. To reflect the hybrid genome of F121-9, two repair templates were generated, one for each allele. These were assembled into pGEM-3Z to make pGEM-3Z-mINTS11-CAST and pGEM-3Z-mINS11-129.

Highlights • New orangutan and chimpanzee iPSC lines enable pan-primate gene expression analysis • Transiently expressed (TrEx) lncRNAs identified in primate cerebral cortex organoids • Conserved TrEx pattern correlates with cell-type specificity by single-cell RNA-seq • Four primate-conserved TrEx lncRNAs influence neural genes when ectopically expressed Summary The cerebral cortex has expanded in size and complexity in primates, yet the molecular innovations that enabled primate-specific...