Bai-bing Zhang

Highlights • Brain endothelial tip cells (ETCs) show local Ca 2+ transients at primary branches • High- and low-frequency Ca 2+ transients distinctly regulate ETC branch dynamics • Mechanosensitive Piezo1 channels mediate local Ca 2+ transients of ETC branches • Mutating piezo1 impairs ETC pathfinding and brain vascular patterning Summary During development, endothelial tip cells (ETCs) located at the leading edge of growing vascular plexus guide angiogenic sprouts to target vessels, and...

(A and B) Loss-of-response rate of zebrafish larvae at 6 dpf in respond to tail-fin pinches. The larvae were treated with different concentration of propofol (A) or etomidate (B) (0, 3, 9, 15, 30, and 100 μM). For each group, 15 larvae were used. The data points for 3 μM propofol or etomidate were totally overlapped with the control group (0 μM). (C) Dose dependency of the loss-of-response rate measured at the eighth minute after the application of propofol or etomidate.