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Barbara Mallon on Muck Rack

Barbara Mallon

As seen in: JoVE- Journal of Visualized Experiments
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Articles

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

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By Anjali Nandal, Barbara Mallon, Bhanu P. Telugu
| JoVE- Journal of Visualized Experiments
In this publication, we have followed a simple but efficient protocol for the generation of iPSC from human pancreatic cells using integration or footprint-free Sendai virus vectors. Figure 1A shows a schematic representation of this reprogramming protocol. The human pancreatic cells were purchased commercially, cultured in Prigrow III medium and transduced with Sendai virus as explained above.
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