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Barbara Tabisz on Muck Rack

Barbara Tabisz

As seen in: JoVE- Journal of Visualized Experiments
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Articles

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

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By Lorenz Meinel, Heike Walles, Claudia Siverino, Barbara Tabisz
| JoVE- Journal of Visualized Experiments
In this article, we describe a method to covalently couple a new BMP2 variant, BMP2-K3Plk, to commercially available azide functionalized agarose beads (Figure 1). The bioactivity of the produced BMP2-K3Plk variant was validated by the induction of alkaline phosphatase (ALP) gene expression in C2C12 cells. The in vitro test shows similar ALP expression levels induced by wild type BMP2 (BMP2-WT) and BMP2-K3Plk (Figure 2).
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