Barbora Salovska

Keywords aneuploidy proteomics dynamic SILAC protein degradation protein synthesis proteostasis gain and loss chromosome 3 lung cancer proteomic buffering Introduction Aneuploidy, defined as the abnormal gain or loss of chromosomes or chromosome arms, is a hallmark of human genetic syndromes and many cancers.1,2,3 While somatic aneuploidy is rare in healthy human tissues,4,5 it is highly prevalent in cancer, with 90% of solid tumors exhibiting aneuploidy.3 This contributes to tumor...

Keywords protein turnover protein lifetime mouse tissues brain regions proteomics pulse SILAC protein phosphorylation DIA-MS TMT Get full text access Log in, subscribe or purchase for full access. References 1. Glass, R.D. ∙ Doyle, D. On the measurement of protein turnover in animal cells J. Biol. Chem. 1972; 247:5234-5242 2. Pratt, J.M. ∙ Petty, J. ∙ Riba-Garcia, I. ... Dynamics of protein turnover, a missing dimension in proteomics Mol. Cell. Proteomics. 2002; 1:579-591 3. Claydon, A.J. ∙ Beynon, R.

Abstract Measuring protein turnover is essential for understanding cellular biological processes and advancing drug discovery. The multiplex DIA mass spectrometry (DIA-MS) approach, combined with dynamic SILAC labeling (pulse-SILAC, or pSILAC), has proven to be a reliable method for analyzing protein turnover and degradation kinetics.