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Circular RNA as a source of neoantigens for cancer vaccines

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By Yi Ren, Thamizhanban Manoharan, Beijia Liu, Bei En Siew
| bmj.com
Clinical samples Healthy donor blood samples were obtained from the Singapore Health Sciences Authority blood bank (Application #202006–06). Human PBMCs were isolated blood samples using Ficoll-Paque (Cytiva, #17144003). The study involved six patients diagnosed with CRC who underwent surgery at National University Hospital. After resection, samples from both tumor and adjacent normal colon tissues were collected and immediately transferred for organoids derivation and DNA/RNA extraction.
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