Bernadette Basilico

Abstract Despite the functional diversity of over 100 causal genes1,2,3, phenotypic convergence across models may reveal common neurobiological processes in autism spectrum disorder (ASD). Here we profiled 251 samples from 11 monogenic mouse models of ASD using single-nucleus multi-omic sequencing across three developmental stages, both sexes and two brain regions. Despite genetic heterogeneity, ASD-linked mutations converged on perturbations of the radial glial cell lineage.

Abstract Neuroimmune signals can regulate neuronal function and affect behavior through mechanisms that are not yet fully understood. Here we investigated the action of interleukin 13 (IL-13), a cytokine that can be produced in the brain by both microglia and neurons. We show that dopamine-containing neurons in the ventral tegmental area (VTA) predominantly express the IL-13 receptor alpha 1 (IL-13Rα1) and exhibit presynaptic vesicular localization of neuronal IL-13.

Antibodies Rat monoclonal Anti-Ctip2 Abcam Cat#ab18465; RRID:AB_2064130 Rabbit polyclonal Anti-Cux1 Santa Cruz Cat#sc-13024; RRID:AB_2261231 Rabbit polyclonal Anti-Iba1 FUJIFILM Wako Cat#019 19741; RRID:AB_839504 Rabbit monoclonal Anti-Gfap Cell Signaling Technologies Cat#12389; RRID:AB_2631098 Mouse monoclonal Anti-Parvalbumin Chemicon Cat#MAB1572;RRID:AB_2174013 Rabbit polyclonal Anti-Cleaved-Caspase-3 Cell Signaling Technologies Cat#9661; RRID:AB_2341188 Rabbit polyclonal Anti Human LAT1...