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Bijentimala Keisham on Muck Rack

Bijentimala Keisham

As seen in: Nature, Cell Press
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Articles

Spatial proximity sequencing maps developmental dynamics in the germinal center

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By Huili Wang, Junjie Xia, Bijentimala Keisham, Abinash Padhi, Yiyu Deng, Luke Vistain, Sarah Kim, Gabriel Mercado-Vásquez, Marcus Clark, Savas Tay
| Cell Press Verified
Keywords spatial sequencing protein-protein interactions protein complexes proximity ligation assay multi-modal germinal center tonsil B cell cell-cell communication multi-omics Get full text access Log in, subscribe or purchase for full access. References 1. Chen, K.H. ∙ Boettiger, A.N. ∙ Moffitt, J.R. ... RNA imaging. Spatially resolved, highly multiplexed RNA profiling in single cells Science. 2015; 348, aaa6090 2. Yao, Z. ∙ van Velthoven, C.T.J. ∙ Kunst, M. ...
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Proximity sequencing for the detection of mRNA, extracellular proteins and extracellular protein complexes in single cells - Nature Protocols

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By Bijentimala Keisham
| Nature Verified
Abstract Complex cellular functions occur via the coordinated formation and dissociation of protein complexes. Functions such as the response to a signaling ligand can incorporate dozens of proteins and hundreds of complexes. Until recently, it has been difficult to measure multiple protein complexes at the single-cell level.
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Quantification of extracellular proteins, protein complexes and mRNAs in single cells by proximity sequencing - Nature Methods

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By Christian Jordi, Mengjie Chen, Bijentimala Keisham
| Nature Verified
Abstract We present proximity sequencing (Prox-seq) for simultaneous measurement of proteins, protein complexes and mRNAs in thousands of single cells. Prox-seq combines proximity ligation assay with single-cell sequencing to measure proteins and their complexes from all pairwise combinations of targeted proteins, providing quadratically scaled multiplexing.
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