Binayak Sarkar

Abstract Progress in immuno-oncology has been stymied by poorly immunogenic ‘cold’ tumours and the focus on T cells at the cost of other immune cells. Here we report that AT-1965, a small molecule in a lipid nanoparticle, induces rapid regression of poorly immunogenic tumours with the formation of immune memory through interaction with Cap-specific RNA (nucleoside-2′-O-)-methyltransferase 2 (CMTR2) in cancer cells, triggering an innate inflammatory viral defence response.

eLife Assessment This valuable study examines the role of IL17-producing Ly6G PMNs as a reservoir for Mycobacterium tuberculosis to evade host killing activated by BCG immunisation. The authors provide solid data reporting that IL17-producing polymorphonuclear neutrophils harbour a significant bacterial load in both wild-type and IFNg-/- mice and that targeting IL17 and Cox2 improved disease outcomes whilst enhancing BCG efficacy.

Request a detailed protocol Virulent laboratory strains of H37Rv, BCG, and GFP-H37Rv bacterial cultures were cultivated on 7H9 medium (BD Difco) supplemented with 10% Oleic Acid-Albumin-Dextrose-Catalase (OADC, BD, Difco), 0.05% glycerol, and 0.05% Tween 80 under shaking at 37°C conditions for in vitro assays. The cultures were then incubated in an orbital shaker at 100 rpm and 37 °C until the mid-log phase.