Byoungkoo Lee

Correction to: Nature Structural & Molecular Biology https://doi.org/10.1038/s41594-025-01708-0, published online 13 January 2026. In the version of this article initially published, Albert W. Cheng should have been designated a co-corresponding author. The error has been corrected in the HTML and PDF versions of the article. About this article Kim, M., Wang, P., Clow, P.A. et al.

Abstract Cohesin is required for chromatin loop formation. However, its precise role in regulating gene transcription remains largely unknown. We investigated the relationship between cohesin and RNA Polymerase II (RNAPII) using single-molecule mapping and live-cell imaging methods in human cells. Cohesin-mediated transcriptional loops were highly correlated with those of RNAPII and followed the direction of gene transcription.

Abstract ChIA-PET (chromatin interaction analysis with paired-end tags) enables genome-wide discovery of chromatin interactions involving specific protein factors, with base pair resolution. Interpretation of ChIA-PET data requires a robust analytic pipeline. Here, we introduce ChIA-PIPE, a fully automated pipeline for ChIA-PET data processing, quality assessment, visualization, and analysis.