What is already known? Noncoplanar polychlorinated biphenyl (PCB) 138, PCB 153, and PCB 180 independently increase lipids stored in adipocytes through distinct mechanisms, and their enzyme induction profiles, nuclear receptor activation, and signaling pathways are congener-specific. Fetal life is a window of susceptibility to environmental obesogens, with consequences for obesity over the life course. What does this study add?