Carrie Eckert

Abstract Cas9-based genome editing technologies can rapidly generate mutations to probe a diverse array of mutant genotypes. However, aberrant Cas9 nuclease translation and activity can occur despite the use of inducible promoters to control expression, leading to extensive cell death. This background killing caused by promoter leakiness severely limits the application of Cas9 for generating mutant libraries because of the potential for population skew.

Glutathione (GSH), a tripeptide composed of l-glutamate, l-glycine and l-cysteine, is the most abundant biological mercaptan in cells [1]. The sulfhydryl group on l-cysteine affects the biological activity of glutathione. GSH (reduction) and GSSG (glutathione disulfide, oxidation) are the two main metabolites [2].

Abstract Bacterial DNA methylation is involved in diverse cellular functions ranging from modulation of gene expression, DNA repair, and restriction-modification systems for defense against viruses and other foreign DNA. Methylome analysis determines sites within a bacterial chromosome that are methylated, revealing motifs that may be targeted by native restriction enzymes.