Cesira de Chiara

Abstract The increasing prevalence of antimicrobial resistance is an important challenge that warrants new approaches to antibiotic development. Currently, all antibiotics inhibit biological processes.

E. coli DdlB (EcDdlB) was co-crystallized, as described in methods, with ATP and D-Ala-D-Ala (Fig. 1a), ADP and D-Ala-D-Ala (Fig. 1b), and ATP and DCS (Fig. 1c) and structures determined at sub 2?Å resolution as summarized in Table 1. The overall structure is described in Supplementary Fig 2.

We carried out a small, targeted version of CoSPI, to demonstrate its ability to uncover non-canonical regulators of enzyme function. By following the formation of phosphoribosyl-ATP (PR-ATP, ? ?=?3600?M?1?cm?1), we tested the effect of 11 commercially available l-His analogues, at a single concentration (1?mM), on the inhibition of ATP-PRT by l-His (Fig. 2a and Supplementary Fig. 4).