Charlene Chan on Muck Rack

Charlene Chan

  • Vice President, Bright Light Marketing
Hawaii
As seen in: Cell Press
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Articles

Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens

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By Charlene Chan, Kleiton Silva Borges, Bradley M. Wierbowski, Jingjing Liu, Jiuchun Zhang, Yunhui Ge, Zhiwen Liu, Daniel Zeve, Ron Weiss, David Breault, Pengxiang Huang, Adrian Salic, Prabhath Mannam
| Cell Press
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Tail-Anchored Protein Insertion by a Single Get1/2 Heterodimer

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By Benjamin E. Zalisko, Charlene Chan, Vladimir Denic, Ronald S. Rock
| Cell Press
To gain insight into the organization of the Get1/2 complex, we developed a bulk fluorescence resonance energy transfer (FRET) assay in proteoliposomes that reports on changes in the proximity of Get1 and/or Get2 subunits upon binding to Get3. We introduced single cysteines at membrane-proximal (Get1-A95C; Get2-E220C) or cytosolic (Get1-S77C; Get2-S28C) positions within Get1 and Get2 (Figure 2Figure 2A). After purification (Figure S1x Download (10.64 MB ) Document S1.
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