Chaya Barbolin

One potential explanation for the high proliferation of B cells is that our SiNET samples may have included germinal centers (GC), in which B cells are expected to proliferate. To assess this possibility, we scored the B cells for a previously defined GC signature (Brescia et al., 2018). While two tumors with high B cell proliferation also had high GC scores, this was not the case for the third tumor (Figure 4—figure supplement 2).

Abstract Single-cell RNA-seq (scRNA-seq) has transformed the study of cancer biology. Recent years have seen a rapid expansion in the number of single-cell cancer studies, yet most of these studies profiled few tumours, such that individual datasets have limited statistical power. Combining the data and results across studies holds great promise but also involves various challenges.

Abstract Neuroendocrine tumors (NETs) occur primarily in the small intestine, lung and pancreas. Due to their rarity compared to other malignancies in these organs, their complex biology remains poorly understood, including their oncogenesis, tumor composition and the intriguing phenomena of mixed neuroendocrine non-neuroendocrine neoplasms (MiNEN). Here we profiled ten low-grade small intestine NET (SiNET) tumor samples as well as one mixed lung tumor by single-cell or single-nuclei RNA-seq.