Christa Geeke Toenhake

Highlights • The ApiAP2 factor AP2-HC is a core component of heterochromatin in malaria parasites • Binding of AP2-HC to heterochromatin strictly depends on heterochromatin protein 1 • The AP2 DNA-binding domain of AP2-HC is dispensable for heterochromatin association Summary Malaria parasites undergo a complex life cycle in the human host and the mosquito vector. The ApiAP2 family of DNA-binding proteins plays a dominant role in parasite development and life cycle progression.

Specific Sequence Motifs Are Associated with Dynamics of Accessible Regions For collections, asynchronous asexual P. falciparum 3D7 cultures were put on ice immediately and filtered over Plasmodipur filters (EuroProxima, Netherlands) to remove human white blood cells. Infected RBCs were washed once in PBS and resuspended in PBS with Protease Inhibitor Cocktail (PI at 1:100, Roche, #04693132001) and 0.05% saponin to a maximum of 6.25% hematocrit for a maximum of 15 minutes.