Christiane Gasperi

STAR★Methods REAGENT or RESOURCESOURCEIDENTIFIER Biological samples Human CSF This study N/A Chemicals, peptides, and recombinant proteins lysyl Endopeptidase®, mass spectrometry grade (Lys-C) FUJIFILM Wako Cat# WAKO129-02541 trypsin, porcine, mass spectrometry grade Sigma Aldrich /Merck Cat# T6567 styroldivinylbenzol-reversed phase sulfonate (SDB-RPS) Empore 3M Cat# 13427427 Isotope labeled heavy spike-in peptides, custom synthesized in manufacturer grade ‘SpikeMix L’ and ‘MaxiSpikeTides L’...

Keywords: multiple sclerosis single-cell sequencing clonal expansion oligoclonal bands neuroinflammation cerebrospinal fluid omics Introduction One of the core features of the vertebrate adaptive immune response is the rapid clonal proliferation of specific lymphocytes on encountering antigen.1 This process is vital to efficiently control infections and malignancy but, when aberrantly activated or inadequately regulated, can result in autoimmune disease.2,3,4 The cerebrospinal fluid (CSF),...

Introduction Pediatric multiple sclerosis (MS) is defined as a disease onset of MS before the age of 18.1 The etiology of MS is multifactorial, involving genetic predisposition and environmental factors.2–4 The potential association between childhood vaccination and the subsequent development of MS has been a subject of considerable debate and research.5,6 Given the critical role of vaccinations in preventing many infectious diseases, it is essential to rigorously assess their safety profile...