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Multifocal Pixel/Photon-Reassignment FLIM (MPPR-FLIM): A Super-Resolution Analytical Tool for Characterizing Subcellular Fluorescence Lifetime Heterogeneity via TCSPC Click to copy article link Article link copied!
Cite Jump to ExpandCollapse ArticleJune 14, 2026 Multifocal Pixel/Photon-Reassignment FLIM (MPPR-FLIM): A Super-Resolution Analytical Tool for Characterizing Subcellular Fluorescence Lifetime Heterogeneity via TCSPC Click to copy article linkArticle link copied! Yongtu Liao Yongtu Liao Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China Danying Lin*...
Resolution-enhanced multifocal structured illumination microscopy using fluorescence fluctuations and Fourier ptychography scheme
Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement over the optical diffraction limit at depths of up to 50 μm in samples. This is achieved through sparse multifocal excitation patterns and digital image post-processing, making MSIM a highly advantageous technique for the three-dimensional super-resolution (SR) imaging of thick specimens. However, the spatial resolution of MSIM is inherently constrained by its underlying imaging principles.
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