Daphne Cornish

You are already at the latest version Viruses have developed a diverse array of mechanisms to hijack host transcriptional machinery and ensure successful viral gene expression. One such method is through manipulation of the cellular machinery required for post-transcriptional processing of host messenger RNAs (mRNAs).

Methods Primary human CD4+ T cells from anonymous, healthy donors were isolated from leukoreduction chambers provided by a commercial vendor (STEMCELL Technologies). These were provided without any identifying information and did not require we attain written informed consent. PBMCs from people living with HIV were provided from study subjects enrolled in the Northwestern University Clinical Research Site for the MACS/WIHS combined cohort study (MWCCS).

Abstract The persistence of HIV-1 in long-lived latent reservoirs during suppressive antiretroviral therapy (ART) remains one of the principal barriers to a functional cure. Blocks to transcriptional elongation play a central role in maintaining the latent state, and several latency reversal strategies focus on the release of positive transcription elongation factor b (P-TEFb) from sequestration by negative regulatory complexes, such as the 7SK complex and BRD4.