Darshan Sapkota

Abstract While circadian rhythm disruption may promote neurodegenerative disease, the impact of aging and neurodegenerative pathology on circadian gene expression patterns in different brain cell types remains unknown. Here we used a translating ribosome affinity purification to identify the circadian translatomes of astrocytes, microglia and bulk tissue in healthy mouse cortex and in the settings of amyloid-β plaque pathology or aging.

Abstract While circadian rhythm disruption may promote neurodegenerative disease, how aging and neurodegenerative pathology impact circadian gene expression patterns in different brain cell types is unknown. Here, we used translating ribosome affinity purification methods to define the circadian translatomes of astrocytes, microglia, and bulk cerebral cortex, in healthy mouse brain and in the settings of amyloid-beta plaque pathology or aging.

New Results View ORCID ProfileDarshan Sapkota, Kristina Sakers, Yating Liu, Allison M Lake, Rohan Khazanchi, Rana R Khankan, Ye Zhang, Joseph D Dougherty doi: https://doi.org/10.1101/2020.04.08.033027 Abstract Gene expression requires two steps-- transcription and translation-- which can be regulated independently to allow nuanced, localized, and rapid responses to cellular stimuli.