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David Adams

Verified
West Palm Beach
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Articles

The structural diversity of telomeres and centromeres across mouse subspecies revealed by complete assemblies

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By David Adams Verified, Michael A. Quail, Jingtao Lilue, Iraad Bonner
| biorxiv.org
Abstract It is over twenty years since the publication of the C57BL/6J mouse reference genome, which has been a key catalyst for understanding mammalian disease biology. However, the mouse reference genome still lacks telomeres and centromeres, contains 281 chromosomal sequence gaps, and only partially represents many biomedically relevant loci. We present the first T2T mouse genomes for two key inbred strains, C57BL/6J and CAST/EiJ.
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Molecular mechanism of plasmid elimination by the DdmDE defense system

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By Luuk Loeff, David Adams Verified, Christelle Chanez, Sandrine Stutzmann
| biorxiv.org
Abstract Seventh pandemic Vibrio cholerae strains contain two hallmark pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryo-EM to reveal the mechanistic basis for plasmid defense by DdmDE. A cryo-EM structure of the DdmD helicase-nuclease reveals that it adopts an auto-inhibited dimeric architecture. The prokaryotic Argonaute protein DdmE uses a DNA guide to target plasmid DNA.
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Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems

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By Alexandre Lemopoulos, David Adams Verified, Melanie Blokesch, Grazia Vizzarro
| biorxiv.org
Abstract In response to predation by bacteriophages and invasion by other mobile genetic elements such as plasmids, bacteria have evolved specialised defence systems that are often clustered together on genomic islands. The O1 El Tor strains of Vibrio cholerae responsible for the ongoing seventh cholera pandemic (7PET) contain a characteristic set of genomic islands involved in host colonisation and disease, many of which contain defence systems.
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