Desiree Macchia

Abstract Analysis of system-wide cellular communication changes in Alzheimer's disease (AD) has recently been enabled by single nucleus RNA sequencing (snRNA-seq) and new computational methods. Here, we combined these to analyze data from postmortem human tissue from the entorhinal cortex of AD patients and compared our findings to those from multiomic data from the 5xFAD amyloidogenic mouse model at two different time points.

Behavioral assays CPP To test for drug-induced CPP, animals were first tested in a single drug pairing, two-chamber CPP test. Each chamber was given different wall contexts. On the first day, animals were initially placed into the right chamber, and allowed to freely explore both chambers for thirty minutes (pre-test). On the second day, animals were saline-conditioned to the left side, and on the following day, cocaine (or morphine)-conditioned to the right side.