Dinah Singer

Plasmid constructs Murine 6X His-BRD4-Flag WT and ΔN, ΔC, ΔB1, ΔB2 and ΔB1B2 mutants are as described previously.6 The BRD4 F1, F2, F3 and F4 fragments are also as described previously.26 The murine BRD4-Flag WT, S1153A, T1222A, T1248A, S1153A+T1222A, T1222A+T1248A and the 3A-BRD4 mutants was generated as point mutants using the TagMaster® Site-Directed Mutagenesis Kit (GM Biosciences) with the BRD4 WT construct in the pFASTbac1 insect vector as template.

The Moonshot has been achieving its intended goals and is rapidly advancing the science for a range of research priorities. March 27, 2020 • By National Cancer Institute By Dinah Singer, PhD Hundreds of investigators participating in the Cancer Moonshot to accelerate cancer research met recently to provide updates on their progress since the initiative was announced in 2016.

February 6, 2019, by NCI Staff During translation, cells make proteins using the genetic information carried in mRNA. A new study shows that an experimental drug can disrupt translation of the PD-L1 protein. Credit: National Cancer Institute Researchers have found an unconventional way to unleash the immune system against liver cancer in mice.