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Dingyi Han

As seen in: Nature, Cell Press
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Articles by Dingyi Han

An engineered, compact Cas12m variant for multiplexed gene activation in vivo

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By Hainan Zhang, Lin Yang, Yuling Liu, Dingyi Han, Yingnan Li, Jiajia Lin, Bingyan Wang, Yaru Zhang, Yi Zhang, Jinhui Li, Shuping Wei, Simiao Liu, Na Zhong, Ganggang Zhang, Xingtao Zhou, Linyu Shi, Jinhai Huang, Hui Yang, Yingsi Zhou, Guoling Li, Jiaxu Hu, Guohe Tan
| Nature Verified
Abstract Achieving coordinated CRISPR-based transactivation (CRISPRa) regulation in vivo remains challenging, as the requisite genetic elements frequently exceed the packaging capacity of AAV vectors. Here we report the identification and engineering of the Cas12m protein family, a naturally nuclease-dead CRISPR system that possesses intrinsic self-processing activity for its precursor crRNA. We develop an optimized variant, denCas12m, and leverage it in the smCRISPRa platform.
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Engineered hypercompact Fanzor-ωRNA system with enhanced genome editing activity

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By Guoli Zhao, Yuling Liu, Ganggang Zhang, Jingjing Wang, Zhilin Zou, Yi Wang, Shuoyu Xu, Dingyi Han, Zhenyu Xu, Zhongxing Chen, Mei Yang, Zhenhai Zeng, Yue Wu, Xingtao Zhou, Jinhai Huang
| Cell Press Verified
Keywords Fanzor SpuFz1 RNA-guided DNA nucleases genome editing protein engineering base editing single-AAV delivery in vivo retinal editing Introduction Programmable nucleases represented by the CRISPR-Cas9 and CRISPR-Cas12 systems enable precise gene editing in target DNA. Among the identified Cas proteins, type II-A Streptococcus pyogenes Cas9 (SpCas9)1,2,3 and type V-A Acidaminococcus sp. BV3L6 Cas12a (AsCas12a)4,5,6 exhibit robust gene editing activity.
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Engineered IscB–ωRNA system with expanded target range for base editing - Nature Chemical Biology

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By Qingquan Xiao, Guoling Li, Haoqiang Wang, Mingyu Yao, Bingbing He, Linyu Shi, Hui Yang, Jinhai Huang, Dingyi Han
| Nature Verified
Abstract As the evolutionary ancestor of Cas9 nuclease, IscB proteins serve as compact RNA-guided DNA endonucleases and nickases, making them strong candidates for base editing. Nevertheless, the narrow targeting scope limits the application of IscB systems; thus, it is necessary to find more IscBs that recognize different target-adjacent motifs (TAMs). Here, we identified 10 of 19 uncharacterized IscB proteins from uncultured microbes with activity in mammalian cells.
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