Elnaz Khalili Samani

1 INTRODUCTION De novo fatty acid synthesis is an evolutionarily conserved process essential to nearly all living organisms, from bacteria to humans (Grininger 2014). Fatty acids produced through this pathway serve critical roles in membrane biogenesis, energy storage, and cellular signaling (Harayama and Riezman 2018).

(A) Cryo-EM map at 2.7 Å resolution of DPYSL2. Density corresponding to each protomer is colored differently. (B) Atomic model of DPYSL2 showing the tetrameric structure of the protein with D2 symmetry.

Abstract Studying native protein structures at near-atomic resolution in crowded environment presents a challenge. Consequently, understanding the structural intricacies of proteins within pathologically affected tissues often relies on mass spectrometry and proteomic analysis.