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Articles by Finn Stirling
Controlling the Implementation of Transgenic Microbes: Are We Ready for What Synthetic Biology Has to Offer?
Adrio J.L. Demain A.L. Recombinant organisms for production of industrial products. Bioeng. Bugs. 2010; 1: 116-131 Agmon N. Tang Z. Yang K. Sutter B. Ikushima S. Cai Y. Caravelli K. Martin J.A. Sun X. Choi W.J. et al. Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 2017; 114: E1470-E1479 Ahrenholtz I. Lorenz M.G. Wackernagel W. A conditional suicide system in Escherichia coli based on the intracellular degradation of DNA. Appl. Environ.
Synthetic Cassettes for pH-Mediated Sensing, Counting, and Containment
Highlights • Containment system with an escape frequency below the detection limit of 10−11 • Evolutionary stability achieved through toxin-antitoxin balancing • Pulse counter developed that responds to the falling edge of a signal • Counter will not advance unless 2 distinct signals are registered Summary As pH is fundamental to all biological processes, pH-responsive bacterial genetic circuits enable precise sensing in any environment.
Rational Design of Evolutionarily Stable Microbial Kill Switches
In the originally published version of this article, there was an error in Figure S3. The sequence in the figure was incorrect due to a copying error in the GenBank file that resulted in a 42 base pair/14 amino acid insertion into the open reading frame of CcdB. This error has since been fixed, and the correct Figure S3 now appears online with the article.
Rational Design of Evolutionarily Stable Microbial Kill Switches
Publication HistoryPublished: November 16, 2017Accepted: October 24, 2017; Received in revised form: August 11, 2017; Received: April 10, 2017;
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