Francesca Cella Zanacchi

SMLM is a potentially helpful tool for quantitative biological experiments. The power of these techniques relies not only on the high spatial resolution achievable, but also on the further possibility of directly accessing quantitative information on a molecular scale (Deschout, Shivanandan, et al., 2014; Jung et al., 2017).

Abstract The use of super-resolution microscopy in recent years has revealed that proteins often form small assemblies inside cells and are organized in nanoclusters. However, determining the copy number of proteins within these nanoclusters constitutes a major challenge because of unknown labeling stoichiometries and complex fluorophore photophysics. We previously developed a DNA-origami-based calibration approach to extract protein copy number from super-resolution images.