Franklin Caval-Holme

Significance Statement Dual-color, real-time imaging is a powerful technique in biomedical imaging, including neuroscience. Here, we present a widely applicable modification to a standard two-photon scanning microscope that adds a near-infrared detection capability, a wavelength range that minimizes photon scattering and autofluorescence from biological samples.

Keywords retinal ganglion cells neuronal types spatial transcriptomics MERFISH perivascular neurons neuroprotection glaucoma neurodegeneration spatial patterning Introduction Cataloging neuronal types, defining their spatial organization, and mapping neuron-neuron connectivity are essential steps for understanding the assembly and function of neural circuits.1,2 The mammalian retina is an ideal model system for these studies due to its compact structure, experimental accessibility, and...