Gongrui Guo

Abstract Alkane monooxygenase (AlkB) is a widely occurring integral membrane metalloenzyme that catalyzes the initial step in the functionalization of recalcitrant alkanes with high terminal selectivity. AlkB enables diverse microorganisms to use alkanes as their sole carbon and energy source. Here we present the 48.6-kDa cryo‐electron microscopy structure of a natural fusion from Fontimonas thermophila between AlkB and its electron donor AlkG at 2.76 Å resolution.

Highlights • BsYetJ mediates Ca2+ flux from the ER in permeabilized mammalian cells • The Ca2+-binding activity is sensitive to protons and other cations • The Asp171-Asp195 dyad controls pore opening and pH-dependent Ca2+ binding • Protonation of Asp171 leads to an open-state structure Summary The anti-apoptotic transmembrane Bax inhibitor motif (TMBIM) containing protein family regulates Ca2+ homeostasis, cell death, and the progression of diseases including cancers.