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Using eDNA sampling for species-specific fish detection in tropical oceanic samples: limitations and recommendations for future use

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By Furlan EM, Hardy CM, Gleeson DM, Gutmann Roberts C
| PeerJ
Primer development CO1 species-specific primers were developed (Table 2) after amplifying tissue from each target species. Primers used to amplify the marker were mlCOIintF (5′-GGWACWGG WTGAACWGTWTAYCCYCC-3′) forward primer and jgHCO2198 (5′-TAIACYTCIGGRTGICCRAARAAYCA-3′) reverse primer to target a 313 bp region of the CO1 marker (Geller et al., 2013; Leray et al., 2013).
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