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Haiying Geng on Muck Rack

Haiying Geng

As seen in: BioRxiv, Pakistan Journal of Medical Sciences
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Articles

Data-driven optimization yielded a highly-efficient CRISPR/Cas9 system for gene editing in Arabidopsis

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By Yifan Wang Verified, Yupu Xu, Yu ZHANG, Haiying Geng
| BioRxiv
Abstract The CRISPR/Cas9 technology is widely used for plant gene editing. In Arabidopsis, the early CRISPR/Cas9 systems using constitutive promoters to drive Cas9 expression usually have low heritable mutation efficiencies in the T1 generation. Germ cell-specific or cell division-specific promoters, like EC1.2en-EC1.1p fusion, YAO and CDC45, have been used as alternatives to direct Cas9 expression, but the efficiency of getting nonchimeric mutations is still not high.
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