Halima Morin

Abstract Gene expression analysis is essential for understanding the mechanisms involved in plant development. Here, we developed M2WISH, a protocol based on MicroWave treatment for Wholemount mRNA In Situ Hybridization in Arabidopsis. By permeabilizing tissues without damaging cellular organisation this protocol results in high and homogeneous hybridization yields that enables systematic analysis of gene expression dynamics.

Díaz A. Zarouri B. Fergany M. Eduardo I. Alvarez J.M. Picó B. Monforte A.J. Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (Cucumis melo L.) [corrected]. PLoS One. 2014; 9: e104188 Périn C. Hagen L.S. Giovinazzo N. Besombes D. Dogimont C. Pitrat M. Genetic control of fruit shape acts prior to anthesis in melon (Cucumis melo L.). Mol. Genet. Genomics. 2002; 266: 933-941 Serquen F.C. Bacher J.

Two allelic j-2 mutants (LA0315 and LA3899) were obtained from the Tomato Genetics Resource Center (TGRC). Both mutants are in determinate genetic backgrounds carrying a mutation in the SELF PRUNING (SP) gene: LA0315 is in Pearson (LA0012) background and LA3899, in addition of sp and j-2 mutations already described in its Ohio8245 parent25, carries mutations B and u accounting for orange and uniform ripening fruits, respectively.