Extracellular vesicles (EVs) derived from microbial sources, including beer yeast (Saccharomyces cerevisiae), have recently attracted increasing attention as bioactive nanostructures with potential biomedical and cosmetic applications. In this study, extracellular vesicles were isolated from Saccharomyces cerevisiae (beer yeast) using an electrokinetic ion-binding filtration system followed by tangential flow filtration (TFF)–based buffer exchange.