Huaitao Cheng

New Results Huaitao Cheng, Han-Pin Pui, Antonio Lentini, Solrun Kolbeinsdottir, Nathanael Andrews, Yu Pei, Bjorn Reinius, Qiaolin Deng, Martin Enge doi: https://doi.org/10.1101/2021.12.02.470912 Abstract Joint single-cell measurements of gene expression and DNA regulatory element activity holds great promise as a tool to understand transcriptional regulation.

Highlights • Scalable and flexible format for joint mRNA and whole-genome sequencing in single cells • RNA and DNA library quality equals single-modality methods • Applied to primary tissue and thousands of artificially altered genomes • Highlights essential genomic regions and transcripts resistant to copy-number change Summary To address how genetic variation alters gene expression in complex cell mixtures, we developed direct nuclear tagmentation and RNA sequencing (DNTR-seq), which...