Hyeshik Chang

Abstract Tandem repeat expansion disorders can be difficult to diagnose when expansions exceed 200 repeats, as standard methods (for example, Southern blot and modified PCR) often fail. We present a Cas9-targeted nanopore sequencing workflow and an automated analysis pipeline, RepeatLab, for accurate repeat-length estimation, structure assessment, and high-resolution methylation profiling.

Abstract mRNA vaccine efficacy depends on sequence optimization, but designing optimal sequences is challenging due to complex cellular RNA regulatory mechanisms. Here we present VaxLab, an open-source web platform that provides the complete design-to-synthesis workflow for mRNA vaccines in a unified interface. VaxLab incorporates four codon optimization algorithms based on distinct approaches: codon usage matching, secondary structure design and deep generative models.

Abstract The limited stability of mRNA in vivo remains a major challenge for vaccines and therapeutics. While alternative RNA formats such as circular RNA or self-amplifying RNA offer greater durability, these modalities often suffer from low translation, modification incompatibility and difficult manufacturing. To overcome these limitations, we screen 196,277 viral sequences and identify eleven elements that strongly enhance mRNA stability and translation.