Hyung Bum Kim

Abstract INTRODUCTION RESULTS DISCUSSION MATERIALS AND METHODS Acknowledgments Supplementary Materials REFERENCES Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Adult women are typically exposed to 17β-estradiol (E2) concentrations of ~100 to 200 pM, yet most cell-based studies use 100 nM. We determined the molecular effects of E2 concentrations spanning six orders of magnitude (1 pM to 100 nM) in breast cancer cells.

Keywords Perturb-seq CRISPR/Cas9 human pluripotent stem cells single-cell genomics cardiomyocytes neural progenitor cells Introduction CRISPR interference (CRISPRi) Perturb-seq is a high-throughput phenotypic assay that combines CRISPRi using a library of single guide RNAs (sgRNAs) with single-cell RNA sequencing (scRNA-seq) to analyze the effects of thousands of genetic perturbations in a single experiment (Replogle et al., 2022; Adamson et al., 2016).

Abstract Estrogens are naturally occurring steroid hormones that also act as the primary mitogens for estrogen receptor-positive (ER+) breast cancers. While elevated blood levels of estrogens have been associated with poor prognosis, the relationship between circulating hormone levels in the blood are related to intracellular hormone concentrations.