Ian Barton

1 INTRODUCTION The proteobacteria are the largest division of prokaryotes which comprises the class α-proteobacteria consisting of a diverse group of bacteria that occupy a wide array of habitats (Batut et al., 2004). Within this class, the agrobacteria, rhizobia, and brucellae represent important plant pathogens, plant symbionts, and mammalian pathogens, respectively.

Abstract Bacteria rely on DNA methylation for restriction-modification systems and epigenetic control of gene expression. Here, we use direct detection of methylated bases by nanopore sequencing to monitor global DNA methylation in Alphaproteobacteria, where use of this technique has not yet been reported.

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