Igor L. Barsukov

eLife Assessment This study provides important insights into how the EBH domain of microtubule end-binding protein 1 (EB1) interacts with SxIP peptides derived from the MACF plus-end tracking protein. The revised manuscript includes convincing ITC and NMR experiments that clarify the role of flanking residues and address the influence of dimerization and cooperativity on binding.

Detection of β–D-glucuronidase activity in environmental samples using 4-fluorophenyl β-D-glucuronide and 19F NMR Common methods for establishing the presence of enteric bacteria polluting water supplies, or in other samples, rely on detecting the hydrolysis of model glucuronide substrates by glucuronidases to release a phenolic product quantifiable by absorbance or fluorescence. Substrates include the β-D-glucuronides of p-nitrophenol, and umbelliferyl or quercetin derivatives.

Abstract The KRAS oncogene drives many common and highly fatal malignancies. These include pancreatic, lung, and colorectal cancer, where various activating KRAS mutations have made the development of KRAS inhibitors difficult.