Ingi Agnarsson

Abstract Evolution in profound darkness often leads to predictable, convergent traits, such as the loss of vision. Yet, the consequences of such repeated evolutionary experiments remain obscure, especially regarding fundamental regulatory behaviors like circadian rhythms. We studied circadian clocks of blind cave spiders and their sighted relatives. In the field, cave spiders exhibit low per expression and maintain constant activity levels.

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Spider sequence data representing all major lineages were collected from previously published transcriptomic and genomic resources ( N = 53) and supplemented with newly sequenced transcriptomes ( N = 21) to form the target taxon set for the current study. Existing sequence data were acquired via the NCBI SRA database ( http://www.ncbi.nlm.nih.gov/sra ). Raw transcriptome sequences were downloaded, converted to fastq file format, and assembled using Trinity ( Grabherr et al., 2011 ).