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Detecting Methylation Changes Induced by Prime Editing

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By Phillip Wong, Shun-Qing Liang, Clifford J Steer, Isaiah Jay Whalen
| Preprints.org
You are already at the latest version While prime editing offers improved precision compared to traditional CRISPR-Cas9 systems, concerns remain regarding potential off-target effects, including epigenetic changes such as DNA methylation. In this study, we investigated whether prime editing induces aberrant CpG methylation patterns. Whole-genome bisulfite sequencing revealed overall methylation similarity between Cas9-edited, and PE2-edited cells.
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