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Izabela Baryła on Muck Rack

Izabela Baryła

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Articles

RNAlater-compatible protocol for nuclei isolation from radical prostatectomy prostate cancer resections, enabling single-nucleus resolution RNA-seq

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By Dorota Anusewicz, Agata Sakowicz, Michał Olczak, Marek Lipinski, Andrzej K. Bednarek, Magdalena Julita Krystkiewicz-Orzechowska, Katarzyna Kośla, Izabela Baryła, Mateusz Mroczek
| Nature Verified
Abstract We present an optimised protocol for single-nucleus isolation from RNAlater-preserved prostate cancer (PC) resections that enables transcriptomically robust snRNA-seq. Our streamlined procedure employs bead-mill homogenization and high-salt lysis to generate nuclei suspensions, eliminating the need for FACS or density-gradient purification. The protocol yielded high-quality libraries (Q30 ≥ 90%) with minimal ambient RNA.
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