Marek Lipinski

Abstract We present an optimised protocol for single-nucleus isolation from RNAlater-preserved prostate cancer (PC) resections that enables transcriptomically robust snRNA-seq. Our streamlined procedure employs bead-mill homogenization and high-salt lysis to generate nuclei suspensions, eliminating the need for FACS or density-gradient purification. The protocol yielded high-quality libraries (Q30 ≥ 90%) with minimal ambient RNA.

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All articles published by MDPI are made immediately available worldwide under an open access license. No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. For more information, please refer to https://www.mdpi.com/openaccess.