Jerker Widengren

Abstract Photobleaching is a general hurdle of fluorescence-based techniques especially in high-resolution microscopy that relies on prolonged and complex illumination. Strategies to reduce photobleaching require chemical modifications of the cell medium, which often compromise physiological cellular conditions.

Nyckelord: NATURVETENSKAP; NATURAL SCIENCES; Biological and Biomedical Physics; Biologisk och biomedicinsk fysik; Sammanfattning: Unparalleled specificity and high sensitivity have made fluorescence microscopy an indispensable tool for life sciences. Both these aspects come from the use of light-emitting fluorophores, labeled to molecules and structures of interest.

Nyckelord: NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Sammanfattning: Various approaches are used in fluorescence-based biological research to enhance the signal-to-background ratio (SBR). One general approach is to move to NIR wavelengths for excitation, where the background from cellular autofluorescence (AF) is minimal. The use of NIR fluorophores offers several additional advantages, including higher penetration depth and lower phototoxicity from lower photon energy.