Jiemin Nah on Muck Rack

Jiemin Nah

As seen in: Cell Press
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Articles

Microprotein SMIM26 drives oxidative metabolism via serine-responsive mitochondrial translation

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By Jiemin Nah, Sreya Mahendran, Baptiste Kerouanton, Liang Cui, Daniella H. Hock, Alfredo Cabrera-Orefice, Kyle Dunlap, David Robinson, Desmond W.H. Tung, Sze Huey Leong, Kiat-Yi Tan, Sonia Chothani, Jingjing Sun, Agnieszka Dziegowska, Grazyna Leszczynska, Owen Rackham, Ilka Wittig, Peter C. Dedon, Gregory S. Ducker, David Stroud, Lena Ho
| Cell Press Verified
SEP-focused CRISPR screen The small ORF-encoded peptide (SEP) focused sgRNA libraries were designed to target 2523 genes, including 201 OXPHOS positive controls, with a total of 12428 guides (average of 4 guides per gene). Oligonucleotides for sgRNAs were synthesized by GenScript, amplified by PCR and cloned into a lentiGuide-Puro (Addgene #52963) vector containing an additional BFP cassette for titering.
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