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Jiying Ning

As seen in: BioRxiv, Cell Press
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Articles

Uncovering synaptic and cellular nanoarchitecture of brain tissue via seamless in situ trimming and milling for cryo-electron tomography

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By Jiying Ning, Jill R Glausier, Rangana Warshamanage, Leslie Gunther-Cummins
| BioRxiv
Abstract Cell-cell communication underlies all emergent properties of the brain, including cognition, learning and memory. The physical basis for these communications is the synapse, a multi-component structure requiring coordinated interactions between diverse cell types. However, many aspects of three-dimensional (3D) synaptic organization remain poorly understood.
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Ribosome-Associated Vesicles promote activity-dependent local translation

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By Eva Martin-Solana, Stephen Carter, Jiying Ning, Eric KF Donahue
| BioRxiv
Abstract Local protein synthesis in axons and dendrites underpins synaptic plasticity. However, the composition of the protein synthesis machinery in distal neuronal processes and the mechanisms for its activity-driven deployment to local translation sites remain unclear. Here, we employed cryo-electron tomography, volume electron microscopy, and live-cell imaging to identify Ribosome-Associated Vesicles (RAVs) as a dynamic platform for moving ribosomes to distal processes.
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Cryo-FIB workflow for imaging brain tissue via in situ cryo-electron microscopy

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By Jiying Ning, Thomas Schmelzer, Jill R Glausier, Chyongere Hsieh
| BioRxiv
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