Johanne E Spreckels

Abstract Early development of the gut ecosystem is crucial for lifelong health. While infant gut bacterial communities have been studied extensively, the infant gut virome remains under-explored. To study the development of the infant gut virome over time and the factors that shape it, we longitudinally assess the composition of gut viruses and their bacterial hosts in 30 women during and after pregnancy and in their 32 infants during their first year of life.

Abstract Human milk microbiome studies are currently hindered by low milk bacterial/human cell ratios and often rely on 16S rRNA gene sequencing, which limits downstream analyses. Here, we aimed to find a method to study milk bacteria and assess bacterial sharing between maternal and infant microbiota. We tested four DNA isolation methods, two bacterial enrichment methods and three sequencing methods on mock communities, milk samples and negative controls.

Role of the gut microbiome in mediating lactose intolerance symptoms http://orcid.org/0000-0001-7918-2481M F Brandao Gois1, Trishla Sinha1, http://orcid.org/0000-0002-8711-1736Johanne E Spreckels1, http://orcid.org/0000-0003-4691-5583Arnau Vich Vila1,2, http://orcid.org/0000-0001-6036-0831Laura A Bolte1,2, http://orcid.org/0000-0001-7928-7371Rinse K Weersma2, Cisca Wijmenga1, Jingyuan Fu1,3, Alexandra Zhernakova1, Alexander Kurilshikov1 1 Department of Genetics, University Medical Centre...